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p aktser473  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc p aktser473
    P Aktser473, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 17202 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+aktser473/Phospho-Akt+(Ser473)+XP+Rabbit+mAb/pm41830859-93-34-36
    Average 99 stars, based on 17202 article reviews
    p aktser473 - by Bioz Stars, 2026-09
    99/100 stars

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    other:

    Article Title: Maternal exercise prevents metabolic disorders in offspring mice through SERPINA3C.
    Article Snippet: Maternal exercise can improve the metabolic health of the offspring.. However, the molecular mechanisms underlying the beneficial effects of maternal exercise on the offspring remain unclear.. Here, we show that maternal exercise during pregnancy alleviates high-fat diet (HFD)-induced adipose inflammation and glucose intolerance in offspring mice, accompanied by upregulation of the adipokine serine protease inhibitor A3C (SERPINA3C) both in maternal adipose tissues and the fetal circulation.

    Article Title: Muscle metabolic resilience and enhanced exercise adaptation by Esr1-induced remodeling of mitochondrial cristae-nucleoid architecture in males
    Article Snippet: p -AKTSer473 , Cell Signaling Technology , 4060; RRID:AB_2315049.

    Saline:

    Article Title: Comparable amino acid & intramuscular signalling responses following consumption of a novel microflora compared to whey protein post-resistance exercise in young adults
    Article Snippet: Proteins were transferred to either a polyvinylidene difluoride or nitrocellulose membrane at 100 V for 1 h in tris-Methanol transfer buffer (Bio-Rad Laboratories, Inc., California, USA). .. Membranes were blocked in 5% low-fat milk (diluted in Tris-buffered saline and 0.1% Tween-20 [TBS-T]) for 1 h at room temperature and then incubated in a rocking device overnight at 4 ◦C in the presence of the following primary antibodies, prepared in Tris Buffered Saline with TBS-T (1:1000): total 4E-BP1 in 5% BSA (CST 9452), total Akt (CST 4691), total eEF2 (CST 2332), total ERK1/2 (CST 4695), total mTOR (CST 2983), total rpS6 (CST 2217), p-4E-BP1Thr37/46 (CST 9459), p-AktSer473 (CST 4060), p-eEF2Thr56 (CST 2331), p-ERK1/2Thr202/Tyr204 (CST 4377), p-mTORSer2448 (CST 2971), p-rpS6Ser235/236 (CST 4858). .. For each, membranes were washed 3 × 5 min in TBS-T, incubated in HRP-linked anti-rabbit (CST 7074, 1:10,000 in TBS-T) at room temperature for 1 h, before 3 × 5 min washes in TBS-T.

    Incubation:

    Article Title: Comparable amino acid & intramuscular signalling responses following consumption of a novel microflora compared to whey protein post-resistance exercise in young adults
    Article Snippet: Proteins were transferred to either a polyvinylidene difluoride or nitrocellulose membrane at 100 V for 1 h in tris-Methanol transfer buffer (Bio-Rad Laboratories, Inc., California, USA). .. Membranes were blocked in 5% low-fat milk (diluted in Tris-buffered saline and 0.1% Tween-20 [TBS-T]) for 1 h at room temperature and then incubated in a rocking device overnight at 4 ◦C in the presence of the following primary antibodies, prepared in Tris Buffered Saline with TBS-T (1:1000): total 4E-BP1 in 5% BSA (CST 9452), total Akt (CST 4691), total eEF2 (CST 2332), total ERK1/2 (CST 4695), total mTOR (CST 2983), total rpS6 (CST 2217), p-4E-BP1Thr37/46 (CST 9459), p-AktSer473 (CST 4060), p-eEF2Thr56 (CST 2331), p-ERK1/2Thr202/Tyr204 (CST 4377), p-mTORSer2448 (CST 2971), p-rpS6Ser235/236 (CST 4858). .. For each, membranes were washed 3 × 5 min in TBS-T, incubated in HRP-linked anti-rabbit (CST 7074, 1:10,000 in TBS-T) at room temperature for 1 h, before 3 × 5 min washes in TBS-T.

    Article Title: Co-Exposure to Bisphenol A and a High-Fat Diet Induces Insulin Resistance via Suppression of Insulin Signaling Molecule Expression and GLUT4 Translocation.
    Article Snippet: .. Proteins were incubated with monoclonal antibodies to AKT (CST, Beverly, MA, USA, 4685, 1:1000), p-AKTSer473 (CST, Beverly, MA, USA, 4060, 1:1000), p-AKTThr308 (CST, Beverly, MA, USA, 13038, 1:1000), Rab8A (Abcam, Cambridge, UK, ab188674, 1:1000), Rab13 (Merck, Darmstadt, Germany, SAB4200057, 1:1000), Syntaxin4 (Abcam, Cambridge, UK, ab184545, 1:1000), VAMP2 (Abcam, Cambridge, UK, ab181869, 1:5000), GLUT4 (Abcam, Cambridge, UK, ab33780, 1:1000), GSK3β (CST, Beverly, MA, USA, 12456, 1:1000), pGSK3βSer9 (CST, Beverly, MA, USA, 9322, 1:1000), and GAPDH (Abcam, Cambridge, UK, ab8245, 1:1000),and subsequently incubated with horseradish https://doi.org/10.3390/toxics14020146 peroxidase-labeled goat anti-rabbit secondary antibodies. .. Bands were developed, and band density was analyzed with ImageJ 1.46r(USA National Institutes of Health).

    Article Title: NLRP3 inflammasome activation and disruption of IRS-1/PI3K/AKT signaling: Potential mechanisms of arsenic-induced pancreatic beta cells dysfunction in rats.
    Article Snippet: Environmental exposure to arsenic is associated with significant health risks, including diabetogenic effects linked to pancreatic dysfunction.. The NOD-like receptor protein 3 (NLRP3) inflammasome has been implicated in various metabolic abnormalities; however, its specific role in arsenic-induced pancreatic dysfunction remains insufficiently understood.. This study aimed to elucidate the involvement and underlying mechanisms of the NLRP3 inflammasome in arsenic-induced pancreatic beta cells dysfunction through in vivo and in vitro models.

    Article Title: MFAP2 promotes metastasis and drug resistance by regulating epithelial-to-mesenchymal transition through EGFR signaling pathway in colorectal cancer cells
    Article Snippet: .. Following blocking 172 with 5% non-fat milk in PBS with 0.02% Tween 20 detergent (PBST) at room 173 temperature for 2 h, the membranes were incubated with primary antibodies, including 174 Jo rna l P repro of MFAP2 (Solarbio, China), GAPDH (BBI Co., Ltd., China), epidermal growth factor 175 receptor (EGFR; Proteintech, China), protein kinase B (AKT) (Proteintech), signal 176 transducer and activator of transcription 3 (STAT3) (Proteintech), and vascular 177 endothelial growth factor A (VEGFA) (Proteintech), p-EGFR (Cell Signaling 178 Technology, USA), p-STAT3 (Cell Signaling Technology), and p-AKTser473(Cell 179 Signaling Technology) antibodies, at 4 °C overnight. .. Horseradish peroxidase-labeled 180 goat anti-rabbit IgG and horseradish peroxidase-labeled goat anti-mouse IgG (BBI Co., 181 Ltd., China) were used for detecting MFAP2 and GAPDH, respectively, after the 182 secondary antibodies were incubated at 37 °C for 2 h. Finally, PVDF membranes were 183 evenly coated with ECL luminescent solution.

    Bioprocessing:

    Article Title: Co-Exposure to Bisphenol A and a High-Fat Diet Induces Insulin Resistance via Suppression of Insulin Signaling Molecule Expression and GLUT4 Translocation.
    Article Snippet: .. Proteins were incubated with monoclonal antibodies to AKT (CST, Beverly, MA, USA, 4685, 1:1000), p-AKTSer473 (CST, Beverly, MA, USA, 4060, 1:1000), p-AKTThr308 (CST, Beverly, MA, USA, 13038, 1:1000), Rab8A (Abcam, Cambridge, UK, ab188674, 1:1000), Rab13 (Merck, Darmstadt, Germany, SAB4200057, 1:1000), Syntaxin4 (Abcam, Cambridge, UK, ab184545, 1:1000), VAMP2 (Abcam, Cambridge, UK, ab181869, 1:5000), GLUT4 (Abcam, Cambridge, UK, ab33780, 1:1000), GSK3β (CST, Beverly, MA, USA, 12456, 1:1000), pGSK3βSer9 (CST, Beverly, MA, USA, 9322, 1:1000), and GAPDH (Abcam, Cambridge, UK, ab8245, 1:1000),and subsequently incubated with horseradish https://doi.org/10.3390/toxics14020146 peroxidase-labeled goat anti-rabbit secondary antibodies. .. Bands were developed, and band density was analyzed with ImageJ 1.46r(USA National Institutes of Health).

    Concentration Assay:

    Article Title: PTEN Loss Promotes PI3Kβ Phosphorylation and EPHA2/SRC/p-PI3KβY962 Complex Assembly to Drive Tumorigenesis
    Article Snippet: .. PI3Kα 711 (Cell Signaling Technology, Cat # 4249, RRID: AB_2165248), PI3Kβ (Cell Signaling 712 Technology, Cat # 3011, RRID: AB_2165246), EPHA2 (Cell Signaling Technology, Cat # 6997, 713 RRID:AB_10827743), p-EPHA2Tyr588 (Cell Signaling Technology, Cat # 12677, RRID: 714 AB_2797989), ERK1/2 (Cell Signaling Technology, Cat # 9102, RRID: AB_330744), 715 p-ERK1/2Tyr202/204 (Cell Signaling Technology, Cat # 9101, RRID: AB_331646), AKT (Cell 716 Signaling Technology, Cat # 4685, RRID: AB_2225340), p-AKTSer473 (Cell Signaling 717 Technology, Cat # 4060, RRID: AB_2315049), PTEN (Cell Signaling Technology, Cat # 2670, 718 RRID: AB_10694066), Pan-Phospho-Tyrosine (Cell Signaling Technology, Cat # 9411, RRID: 719 AB_331228), SRC (Cell Signaling Technology, Cat # 2109, RRID: AB_2106059), p-SRCY416 720 (Cell Signaling Technology, Cat # 6943, RRID: AB_10013641), GST (Cell Signaling 721 Technology, Cat # 2622, RRID: AB_331670), c-Myc (Abcam, Cat # ab178430, RRID: 722 AB_2892202), p-c-MycSer62 (Cell Signaling Technology, Cat # 13748, RRID: AB_2687518), 723 D ow nloaded from http://aacrjournals.org/cancerdiscovery/article-pdf/doi/10.1158/2159-8290.C D -25-1126/3670744/cd-25-1126.pdf by guest on 16 N ovem ber 2025 Ki67 (Abcam, Cat # ab16667, RRID: AB_302459), PI3Kβ (Mouse) (Proteintech, Cat # 724 67121-1-Ig, RRID: AB_2882424), Flag (Abmart, Cat # M20008, RRID: AB_2713960), HA 725 (Abmart, Cat # M20003, RRID: AB_2864345), Myc (Abmart, Cat # M20002, RRID: 726 AB_2861172), ACTIN (GenScript, Cat # A00702-100, RRID: AB_914102), anti-rabbit 727 secondary antibodies (GenScript, Cat # A00098, RRID: AB_1968815), anti-mouse secondary 728 antibodies (GenScript, Cat # A00160, RRID: AB_1968937), Alexa Fluor Plus 488 donkey 729 anti-mouse IgG secondary antibody (Invitrogen, Cat # A32766TR, RRID: AB_2866493), 730 Alexa Fluor Plus 555 donkey anti-rabbit IgG secondary antibody (Invitrogen, Cat # A32794, 731 RRID: AB_2762834) 732 733 Dot-blot assays 734 Dilute the synthetic peptides (ILTYDFIHC) phosphorylated peptides (ILTY962piDFIHC) to 735 an appropriate concentration. ..

    Blocking Assay:

    Article Title: MFAP2 promotes metastasis and drug resistance by regulating epithelial-to-mesenchymal transition through EGFR signaling pathway in colorectal cancer cells
    Article Snippet: .. Following blocking 172 with 5% non-fat milk in PBS with 0.02% Tween 20 detergent (PBST) at room 173 temperature for 2 h, the membranes were incubated with primary antibodies, including 174 Jo rna l P repro of MFAP2 (Solarbio, China), GAPDH (BBI Co., Ltd., China), epidermal growth factor 175 receptor (EGFR; Proteintech, China), protein kinase B (AKT) (Proteintech), signal 176 transducer and activator of transcription 3 (STAT3) (Proteintech), and vascular 177 endothelial growth factor A (VEGFA) (Proteintech), p-EGFR (Cell Signaling 178 Technology, USA), p-STAT3 (Cell Signaling Technology), and p-AKTser473(Cell 179 Signaling Technology) antibodies, at 4 °C overnight. .. Horseradish peroxidase-labeled 180 goat anti-rabbit IgG and horseradish peroxidase-labeled goat anti-mouse IgG (BBI Co., 181 Ltd., China) were used for detecting MFAP2 and GAPDH, respectively, after the 182 secondary antibodies were incubated at 37 °C for 2 h. Finally, PVDF membranes were 183 evenly coated with ECL luminescent solution.



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